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A fluorescence in situ hybridisation (FISH) assay has been used to screen for ETV1 gene rearrangements in a cohort of 429 prostate cancers from patients who had been diagnosed by trans-urethral resection of the prostate. The presence of ETV1 gene alterations (found in 23 cases, 5.4%) was correlated with higher Gleason Score (P=0.001), PSA level at diagnosis (P=<0.0001) and clinical stage (P=0.017) but was not linked to poorer survival. We found that the six previously characterised translocation partners of ETV1 only accounted for 34% of ETV1 re-arrangements (eight out of 23) in this series, with fusion to the androgen-repressed gene C15orf21 representing the commonest event (four out of 23). In 5'-RACE experiments on RNA extracted from formalin-fixed tissue we identified the androgen-upregulated gene ACSL3 as a new 5'-translocation partner of ETV1. These studies report a novel fusion partner for ETV1 and highlight the considerable heterogeneity of ETV1 gene rearrangements in human prostate cancer.

Original publication

DOI

10.1038/sj.bjc.6604472

Type

Journal article

Journal

Br J Cancer

Publication Date

22/07/2008

Volume

99

Pages

314 - 320

Keywords

Coenzyme A Ligases, Cohort Studies, DNA-Binding Proteins, Gene Fusion, Gene Rearrangement, Genetic Heterogeneity, Humans, In Situ Hybridization, Fluorescence, Male, Paraffin Embedding, Prostatic Neoplasms, Reverse Transcriptase Polymerase Chain Reaction, Transcription Factors, Translocation, Genetic