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The monoclonal anti-CD20 antibody rituximab (RTX) depletes B cells in the treatment of lymphoma and autoimmune disease, and contributes to alloantibody reduction in transplantation across immunologic barriers. The effects of RTX on T cells are less well described. T-follicular helper (Tfh) cells provide growth and differentiation signals to germinal center (GC) B cells to support antibody production, and suppressive T-follicular regulatory (Tfr) cells regulate this response. In mice, both Tfh and Tfr are absolutely dependent on B cells for their formation and on the GC for their maintenance. In this study, we demonstrate that RTX treatment results in a lack of GC B cells in human lymph nodes without affecting the Tfh or Tfr cell populations. These data demonstrate that human Tfh and Tfr do not require an ongoing GC response for their maintenance. The persistence of Tfh and Tfr following RTX treatment may permit rapid reconstitution of the pathological GC response once the B-cell pool begins to recover. Strategies for maintaining remission after RTX therapy will need to take this persistence of Tfh into account.

Original publication

DOI

10.1182/blood-2014-07-585976

Type

Journal article

Journal

Blood

Publication Date

23/10/2014

Volume

124

Pages

2666 - 2674

Keywords

Adolescent, Adult, Aged, Antibodies, Monoclonal, Murine-Derived, Antibody Formation, Antigens, CD19, B-Lymphocytes, CD57 Antigens, Cell Differentiation, Cells, Cultured, Coculture Techniques, Female, Flow Cytometry, Germinal Center, Humans, Immunologic Factors, Lymph Nodes, Lymphocyte Count, Male, Middle Aged, Receptors, CXCR5, Rituximab, T-Lymphocytes, Helper-Inducer, T-Lymphocytes, Regulatory, Tumor Necrosis Factor Receptor Superfamily, Member 7, Young Adult