3D pathology-guided microdissection.
Hsieh H-C., Gao G., Han Q., Brenes D., Baraznenok E., Yan R., Serafin R., Bishop KW., Wang R., Konnick EQ., Pritchard CC., Figiel S., Hamdy FC., Mills IG., Reder NP., Reddi DM., Paulson TG., Grady WM., Valk JE., True LD., Haffner MC., Rao SR., Woodcock DJ., Liu JTC.
Traditional micro- and macrodissection techniques enable the extraction of localized regions in thin tissue sections for molecular analysis. Despite the growing use of three-dimensional (3D) microscopy, analogous methods for volumetric microdissection are lacking. Here we have developed a 3D microdissection method based on computer numerical controlled milling integrated with open-top light-sheet microscopy. We demonstrate the ability to study tumor evolution along convoluted 3D branching architectures, which is inaccessible to two-dimensional methods.