Malignant pleural mesothelioma (MPM) is an aggressive cancer, associated with poor prognosis. We assessed the feasibility of patient-derived cell cultures to serve as an ex vivo model of MPM. Patient-derived MPM cell cultures (n=16) exhibited stemness features and reflected intratumour and interpatient heterogeneity. A subset of the cells were subjected to high-throughput drug screening and coculture assays with cancer-specific cytotoxic T cells and showed diverse responses. Some of the biphasic MPM cells were capable of processing and presenting the neoantigen SSX-2 endogenously. In conclusion, patient-derived MPM cell cultures are a promising and faithful ex vivo model of MPM.
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mesothelioma, pleural disease, Biomarkers, Tumor, Cell Culture Techniques, Genes, Tumor Suppressor, High-Throughput Screening Assays, Humans, Immunotherapy, Mesothelioma, Malignant, Mutation, Pleural Neoplasms, Tumor Cells, Cultured, Whole Genome Sequencing