Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Dendritic cells (DC) are specialized antigen-presenting cells. DC can acquire and process antigens in the periphery before maturing and migrating to secondary lymphoid tissues where they present the antigens and deliver co-stimulatory signals to T cells. We describe an immunostimulatory oligonucleotide containing a CpG motif that stimulated murine DC to up-regulate co-stimulatory molecules, induce T-cell proliferative responses and secrete interleukin-12 in vitro. Administration of this oligonucleotide, but not of a control oligonucleotide lacking this motif, to mice led to the disappearance of DC from the marginal zone and T-cell areas of spleen, but not from heart or kidney. The same CpG did not cause maturation of monocyte-derived human DC in vitro, but lipopolysaccharide-treated monocyte-derived DC showed enhanced functional activity and up-regulated co-stimulatory molecules.

Type

Journal article

Journal

Immunology

Publication Date

03/2000

Volume

99

Pages

361 - 366

Keywords

Animals, Antigens, CD, Antigens, CD40, Antigens, CD86, Cell Movement, Cells, Cultured, CpG Islands, Dendritic Cells, Female, Flow Cytometry, Histocompatibility Antigens Class II, Humans, Immunohistochemistry, Integrin alphaXbeta2, Interleukin-12, Lectins, C-Type, Lipopolysaccharides, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Minor Histocompatibility Antigens, Oligonucleotides, Receptors, Cell Surface, Spleen