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The mouse is a widely used model for investigating the pathophysiological and genetic bases of otitis media (OM). It has proven a valuable tool for investigating the multifactorial bases of OM including the role of pathogens, anatomical factors, inflammatory mediators and susceptibility loci. However, straightforward and robust phenotyping tools for identifying murine otitis media are lacking, which has precluded for example the identification of mice with OM in genetic screens without resorting to time-consuming histopathology. We have set out to develop a phenotyping platform for the detection of OM in mice utilizing oto-endoscopy. We have applied the technique to a cohort of mice genetically susceptible to chronic otitis media. We show that oto-endoscopy is a safe, reliable and valid method for detecting otitis media in the mouse and discuss its utility in screens to identify novel genes involved with susceptibility to OM.

Original publication

DOI

10.1016/j.heares.2010.09.002

Type

Journal article

Journal

Hear Res

Publication Date

02/2011

Volume

272

Pages

5 - 12

Keywords

Animals, Diagnostic Techniques, Otological, Disease Models, Animal, Ear, Middle, Endoscopy, Equipment Design, Genetic Predisposition to Disease, Mice, Mice, Mutant Strains, Mutation, Otitis Media, Phenotype, Proteins, Reproducibility of Results, Smad2 Protein, Smad3 Protein, Tumor Suppressor Protein p53