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We have reported that murine leukemia cell line (C2M-A5) induced apoptosis by G-CSF. To clarify the mechanism, mRNA expression of apoptosis-related genes was studied. It revealed transient over-expression of c-myc, H-ras and p53 and down-expression of bcl-2. These changes were known as triggers of endonuclease induction. After 96 h culture with G-CSF, apoptosis was occurred simultaneously with endonuclease (37 kd) activation. This endonuclease induced the digestion of double-strand DNA and might be associated with caspase3. Although G-CSF accelerates cell growth and prevents apoptosis in general, it is a contradictory effect. We concluded that G-CSF induced endogenous endonuclease activity in C2M-A5.

Type

Journal article

Journal

Leuk Res

Publication Date

12/2000

Volume

24

Pages

1033 - 1039

Keywords

Animals, Apoptosis, Caspase 3, Caspases, Endonucleases, Gene Expression, Genes, bcl-2, Genes, myc, Genes, p53, Genes, ras, Granulocyte Colony-Stimulating Factor, Leukemia, Myeloid, Mice, RNA, Messenger, Tumor Cells, Cultured