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The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.

Original publication

DOI

10.1002/elps.200406094

Type

Journal article

Journal

Electrophoresis

Publication Date

11/2004

Volume

25

Pages

3769 - 3777

Keywords

Biomarkers, Carrier Proteins, Chromatography, Affinity, Glycoproteins, Humans, Male, Microarray Analysis, Microspheres, Prostate-Specific Antigen, Prostatic Diseases, Prostatic Neoplasms, Proteins, Semen, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization