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Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5ngmL-1, 80pgmL-1, and 800fgmL-1 when arraying the PSA antibody, H117 at the concentration 15μgmL-1, 35μgmL-1, and 154μgmL-1, respectively. We further investigated PSA spiked into human female serum in the range of 800fgmL-1 to 500ngmL-1. The microarray showed a LOD of 800fgmL-1 and a dynamic range of 800fgmL-1 to 80ngmL-1 in serum spiked samples. © 2013 Elsevier B.V.

Original publication

DOI

10.1016/j.aca.2013.06.041

Type

Journal article

Journal

Analytica Chimica Acta

Publication Date

24/09/2013

Volume

796

Pages

108 - 114