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To address immunocapture of proteins in large cohorts of clinical samples high throughput sample processing is required. Here a method using the proteomic sample platform, ISET (integrated selective enrichment target) that integrates highly specific immunoaffinity capture of protein biomarker, digestion and sample cleanup with a direct interface to mass spectrometry is presented. The robustness of the on-ISET protein digestion protocol was validated by MALDI MS analysis of model proteins, ranging from 40 fmol to 1 pmol per nanovial. On-ISET digestion and MALDI MS/MS analysis of immunoaffinity captured disease-associated biomarker PSA (prostate specific antigen) from human seminal plasma are presented.

Original publication




Journal article


Anal Chim Acta

Publication Date





1 - 8


Digestion, ISET, Immunoaffinity, MALDI, MRM, MS, PSA, Proteomics, RP, SPE, SPR, integrated selective enrichment target, mass spectrometry, matrix-assisted laser desorption/ionization, multiple reaction monitoring, prostate specific antigen, reverse phase, solid-phase extraction, surface plasmon resonance, Alkylation, Antibodies, Monoclonal, Chromatography, Affinity, Humans, Male, Oxidation-Reduction, Prostate-Specific Antigen, Proteomics, Solid Phase Extraction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization