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The isolation and transplantation of porcine islets represent a future option for the treatment of type 1 diabetic patients. Stringent product release criteria and limited availability of transgenic and specific pathogen-free pigs will essentially require processing of explanted pig pancreata in specialized, possibly remote isolation facilities, whereby pancreata are exposed to cold ischemia due to prolonged tissue transit time. In the present study we investigated whether pancreas oxygenation can be efficiently combined with an antioxidant strategy utilizing intraductal L-glutamine administration. Pig pancreata were intraductally perfused after retrieval and after cold storage in oxygen-precharged perfluorohexyloctane utilizing University of Wisconsin solution supplemented with (n = 16) or without (n = 14) 5 mmol/L L-glutamine. After isolation purified islets were subjected to extensive quality assessment. Islet recovery postpurification was significantly higher in glutamine-treated pancreata (77.0 ± 3.3% vs. 60.3 ± 6.0%, p 

Original publication




Journal article


Cell Transplant

Publication Date





531 - 538


Adenosine, Allopurinol, Animals, Cold Ischemia, Female, Glutamine, Glutathione, Inflammation, Inflammation Mediators, Insulin, Islets of Langerhans, Islets of Langerhans Transplantation, Mice, Nude, Organ Preservation, Organ Preservation Solutions, Protective Agents, Raffinose, Reactive Oxygen Species, Swine