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Tumour cells sustain their high proliferation rate through metabolic reprogramming, whereby cellular metabolism shifts from oxidative phosphorylation to aerobic glycolysis, even under normal oxygen levels. Hypoxia-inducible factor 1A (HIF1A) is a major regulator of this process, but its activation under normoxic conditions, termed pseudohypoxia, is not well documented. Here, using an integrative approach combining the first genome-wide mapping of chromatin binding for an endocytic adaptor, ARRB1, both in vitro and in vivo with gene expression profiling, we demonstrate that nuclear ARRB1 contributes to this metabolic shift in prostate cancer cells via regulation of HIF1A transcriptional activity under normoxic conditions through regulation of succinate dehydrogenase A (SDHA) and fumarate hydratase (FH) expression. ARRB1-induced pseudohypoxia may facilitate adaptation of cancer cells to growth in the harsh conditions that are frequently encountered within solid tumours. Our study is the first example of an endocytic adaptor protein regulating metabolic pathways. It implicates ARRB1 as a potential tumour promoter in prostate cancer and highlights the importance of metabolic alterations in prostate cancer.

Original publication

DOI

10.15252/embj.201386874

Type

Journal article

Journal

EMBO J

Publication Date

17/06/2014

Volume

33

Pages

1365 - 1382

Keywords

Adaptor, hypoxia, metabolism, prostate, transcription, Arrestins, Chromatin Immunoprecipitation, Fluorescent Antibody Technique, Fumarate Hydratase, Gas Chromatography-Mass Spectrometry, Gene Expression Profiling, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Immunoblotting, Immunohistochemistry, Magnetic Resonance Spectroscopy, Male, Metabolic Networks and Pathways, Metabolomics, Models, Biological, Prostatic Neoplasms, RNA Interference, Succinate Dehydrogenase, Tissue Array Analysis, beta-Arrestin 1, beta-Arrestins