Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Dendritic cells (DC) are central to the control of adaptive immunity. Their ability to activate antigen-specific T cells depends on their maturation state. Many microbial and inflammatory products have stimulated DC maturation. This in vitro study used assays of phenotype and function to examine the potential of bacillus Calmette-Guerin, muramyl dipeptide, and CpG-rich oligodeoxynucleotides to stimulate DC maturation. A chemical fixation method was developed to reliably assess the functional potential of stimulated DC within a mixed lymphocyte reaction model. Using this method, it was shown that bacillus Calmette-Guerin provides a maturation signal as effective as the prototype DC stimulant interleukin-1beta. Furthermore, weaker stimuli such as muramyl dipeptide and CpG-rich oligodeoxynucleotides also are able to induce functional maturation of DC. Using chemical fixation, it was possible to generate stable DC in an immature or a mature state. These observations have importance for our understanding of the regulation of adaptive immunity and for the design of DC-based immunotherapeutic strategies.

Type

Journal article

Journal

J Immunother

Publication Date

03/2002

Volume

25

Pages

152 - 161

Keywords

Acetylmuramyl-Alanyl-Isoglutamine, CD4-Positive T-Lymphocytes, Cell Differentiation, Dendritic Cells, Fixatives, Gamma Rays, Humans, Immunotherapy, In Vitro Techniques, Interleukin-1, Lymphocyte Activation, Lymphocyte Culture Test, Mixed, Models, Immunological, Mycobacterium bovis, Oligodeoxyribonucleotides