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PURPOSE: To study the c-erbB-2 oncogene in primary transitional cell bladder cancer. MATERIALS AND METHODS: Ninety-five patients with known clinical follow-up and epidermal growth factor receptor (EGFr) status were studied for expression of c-erbB-2 by immunostaining. Possible mechanisms underlying increased staining for c-erbB-2 protein were investigated by analyzing DNA and RNA encoding c-erbB-2. RESULTS: Strong positive staining for c-erbB-2 was detected in 20 (21%) tumors, with weaker staining in a further 13 (14%). There was no correlation between increased staining for c-erbB-2 and tumor stage, grade, or EGFr status. There was a low rate of amplification of the c-erbB-2 gene (1 of 24) on Southern blotting with a higher rate of elevated c-erbB-2 mRNA (4 of 44) with dot blot hybridization. For pT1 tumors, the rate of recurrence was higher for those tumors which were positive for c-erbB-2. CONCLUSIONS: c-erbB-2 oncoprotein is expressed by a significant proportion of transitional cell tumors of the bladder. In this study, the prognostic significance of c-erbB-2 expression appears limited.

Type

Journal article

Journal

J Urol

Publication Date

01/1996

Volume

155

Pages

321 - 326

Keywords

Blotting, Southern, Carcinoma, Transitional Cell, DNA, Neoplasm, Gene Expression, Genes, erbB-2, Humans, Immunoblotting, Neoplasm Recurrence, Local, Oncogene Proteins v-erbB, Prognosis, RNA, Messenger, RNA, Neoplasm, Receptor, Epidermal Growth Factor, Urinary Bladder, Urinary Bladder Neoplasms