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We have identified an HLA-B*07 variant allele, B*0716, in a Caucasoid cadaver kidney donor. The HLA class I type by polymerase chain reaction using sequence-specific primers (PCR-SSP) was A*01, 32; B*07, 08; Cw*07. Serological typing, using monoclonal and polyclonal anti-HLA antisera, gave disparate results for the B antigens. Monoclonal antibodies identified B7 and B8 antigens but polyclonal antisera recognised only the B8 antigen. PCR using sequencing based typing (PCR-SBT) confirmed the presence of both B*0703 and B*0801 alleles but with a mutation in one of the alleles. The HLA-B*07 allele was isolated by allele-specific PCR and was shown to have a mutation, G-->T, at 292 in exon 2. This mutation changes codon 74, which encodes aspartic acid (GAC) present in all previously identified B*07 alleles, to tyrosine (TAC) in the variant. The serological results suggest that codon 74 is a crucial part of a B7 antigen-specific epitope recognised by tissue typing polyclonal antisera.

Type

Journal article

Journal

Tissue Antigens

Publication Date

01/2000

Volume

55

Pages

71 - 73

Keywords

Alleles, Amino Acid Sequence, Base Sequence, DNA Primers, Epitopes, Genes, MHC Class I, HLA-B7 Antigen, Histocompatibility Testing, Humans, Molecular Sequence Data, Point Mutation, Polymerase Chain Reaction, Sequence Analysis, DNA, Serotyping