My research interest is to reduce hypoxia-induced damage in human islets of Langerhans during pancreas procurement, after isolation and subsequent to transplantation.
During the last years we identified perfluorohexyloctane as an efficient oxygen carrier for human pancreas preservation. This substance is characterised by increased lipophilicity and lower gravity compared to already established compounds and recently obtained approval for clinical use. We are currently aiming to develop an emulsion that combines the characteristics of both hyperoxygen carriers and organ preservation solutions.
In contrast to whole pancreases isolated islets can easily be treated during cell culture to increase their resistance toward hypoxia-induced damage prior to and after transplantation into recipients. Currently, we investigate whether islet susceptiblity toward hypoxia can be reduced by inducing intrinsic mechanisms for stress resistance or by using potentially protective substances.
Strategies to improve the oxygen supply to microencapsulated islets.
Brandhorst D. et al, (2019), Transplantation
Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation.
Brandhorst H. et al, (2018), Cell Transplant
Does Islet Size Really Influence Graft Function After Clinical Islet Transplantation?
Hughes SJ. et al, (2018), Transplantation, 102, 1857 - 1863
Effect of Etanercept Concentration on Human Islet Integrity.
Acreman S. et al, (2017), Transplant Proc, 49, 2327 - 2329
Hypoxia-Induced Damage in Human Islets Is Reduced With the Use of Mesenchymal Stem Cell-Preconditioned Medium.
Brandhorst D. et al, (2017), Transplant Proc, 49, 2330 - 2332