Daniel Brandhorst

• Anti-caspase-3 preconditioning increases proinsulin secretion and deteriorates posttransplant function of isolated human islets.

  Brandhorst D. et al, (2013), Apoptosis, 18, 681 - 688

• Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.

  Brandhorst H. et al, (2013), Cell Transplant, 22, 1477 - 1483

• Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs.

  Asif S. et al, (2012), J Surg Res, 178, 959 - 967

• Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs

  Asif S. et al, (2012), Journal of Surgical Research, 178, 959 - 967

• Using HTK for prolonged pancreas preservation prior to human islet isolation.

  Caballero-Corbalán J. et al, (2012), J Surg Res, 175, 163 - 168

• Using HTK for prolonged pancreas preservation prior to human islet isolation

  Caballero-Corbalán J. et al, (2012), Journal of Surgical Research, 175, 163 - 168

• Chemical Oxygenation of Pancreatic Tissue Prior to Islet Isolation and Transplantation

  Brandhorst H. et al, (2012), ORGAN DONATION AND TRANSPLANTATION – PUBLIC POLICY AND CLINICAL PERSPECTIVES, 217 - 238

• Quantification of the islet product: presentation of a standardized current good manufacturing practices compliant system with minimal variability.

  Friberg AS. et al, (2011), Transplantation, 91, 677 - 683

• Long-term engraftment and function of transplanted pancreatic islets in vascularized segments of small intestine.

  Kakabadze Z. et al, (2011), Transpl Int, 24, 175 - 183

• Large-scale bioreactor expansion of tumor-infiltrating lymphocytes.

  Sadeghi A. et al, (2011), J Immunol Methods, 364, 94 - 100

• Pathogen inactivation of human serum facilitates its clinical use for islet cell culture and subsequent transplantation.

  Ståhle MU. et al, (2011), Cell Transplant, 20, 775 - 781

• The effect of truncated collagenase class I isomers on human islet isolation outcome.

  Brandhorst H. et al, (2010), Transplantation, 90, 334 - 335

• Mammalian tissue-free liberase: a new GMP-graded enzyme blend for human islet isolation.

  Caballero-Corbalán J. et al, (2010), Transplantation, 90, 332 - 333

• A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.

  Brandhorst H. et al, (2010), Transplantation, 89, 155 - 160

• Large-scale comparison of Liberase HI and collagenase NB1 utilized for human islet isolation.

  Brandhorst H. et al, (2010), Cell Transplant, 19, 3 - 8

• Vitacyte collagenase HA: a novel enzyme blend for efficient human islet isolation.

  Caballero-Corbalán J. et al, (2009), Transplantation, 88, 1400 - 1402

• Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation.

  Brandhorst H. et al, (2009), Transpl Int, 22, 1017 - 1022

• The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation.

  Brandhorst H. et al, (2009), Transplantation, 87, 370 - 375

• Pig pancreas oxygenation at 20 degrees C increases islet ATP generation but deteriorates islet function.

  Iken M. et al, (2009), Cell Transplant, 18, 745 - 751

• Dissecting the instant blood-mediated inflammatory reaction in islet xenotransplantation (Xenotransplantation 15, 225-234)

  Goto M. et al, (2008), Xenotransplantation, 15

• Dissecting the instant blood-mediated inflammatory reaction in islet xenotransplantation.

  Goto M. et al, (2008), Xenotransplantation, 15, 225 - 234

• New class of oxygen carriers improves islet isolation from long-term stored rat pancreata.

  Brandhorst H. et al, (2008), Transplant Proc, 40, 393 - 394

• Degraded collagenase deteriorates islet viability.

  Brandhorst H. et al, (2008), Transplant Proc, 40, 370 - 371

• The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas.

  Brandhorst H. et al, (2008), Transplantation, 85, 456 - 461

• Human islet separation utilizing a closed automated purification system.

  Friberg AS. et al, (2008), Cell Transplant, 17, 1305 - 1313

• Positron emission tomography: a real-time tool to quantify early islet engraftment in a preclinical large animal model.

  Eich T. et al, (2007), Transplantation, 84, 893 - 898

• No beneficial effect of two-layer storage compared with UW-storage on human islet isolation and transplantation.

  Caballero-Corbalán J. et al, (2007), Transplantation, 84, 864 - 869

• Effect of pretransplant preconditioning by whole body hyperthermia on islet graft survival.

  Brandhorst H. et al, (2007), Cell Transplant, 16, 707 - 715

• Pancreas storage in oxygenated perfluorodecalin does not restore post-transplant function of isolated pig islets pre-damaged by warm ischemia.

  Brandhorst D. et al, (2006), Xenotransplantation, 13, 465 - 470

• Ischaemia is linked to inflammation and induction of angiogenesis in pancreatic islets.

  Linn T. et al, (2006), Clin Exp Immunol, 144, 179 - 187

• Induction and amelioration of environmental stress in isolated islets until transplantation

  Brandhorst D. et al, (2006), Immunology, Endocrine and Metabolic Agents in Medicinal Chemistry, 6, 206 - 218

• Oxygen and pancreas preservation.

  Mirbolooki M. and Lakey JR., (2006), Transplantation, 81

• Porcine islet graft function is affected by pretreatment with a caspase-3 inhibitor.

  Brandhorst D. et al, (2006), Cell Transplant, 15, 311 - 317

• Adiponectin is functionally active in human islets but does not affect insulin secretory function or beta-cell lipoapoptosis.

  Staiger K. et al, (2005), J Clin Endocrinol Metab, 90, 6707 - 6713

• Effect of stable glutamine compounds on porcine islet culture.

  Brandhorst H. et al, (2005), Transplant Proc, 37, 3519 - 3520

• Adjustment of the ratio between collagenase class II and I improves islet isolation outcome.

  Brandhorst D. et al, (2005), Transplant Proc, 37, 3450 - 3451

• Vascular endothelial growth factor increases functional beta-cell mass by improvement of angiogenesis of isolated human and murine pancreatic islets.

  Lai Y. et al, (2005), Transplantation, 79, 1530 - 1536

• Successful pancreas preservation by a perfluorocarbon-based one-layer method for subsequent pig islet isolation.

  Brandhorst D. et al, (2005), Transplantation, 79, 433 - 437

• Influence of collagenase loading on long-term preservation of pig pancreas by the two-layer method for subsequent islet isolation.

  Brandhorst D. et al, (2005), Transplantation, 79, 38 - 43

• Timing of hyperthermic preconditioning affects islet resistance against inflammation.

  Brandhorst D. et al, (2005), Transplant Proc, 37, 231 - 232

• The ratio between class II and class I collagenase determines the amount of neutral protease activity required for efficient islet release from the rat pancreas.

  Brandhorst H. et al, (2005), Transplant Proc, 37, 215 - 216

• Long-term preservation of the pig pancreas by a one-layer method for successful islet isolation.

  Brandhorst D. et al, (2005), Transplant Proc, 37, 229 - 230

• Influence of neutral protease activity on human islet isolation outcome.

  Brandhorst H. et al, (2005), Transplant Proc, 37, 241 - 242

• Adaption of neutral protease activity for islet isolation from the long-term two-layer method-stored pig pancreas.

  Brandhorst D. et al, (2005), Transplant Proc, 37, 458 - 459

• Optimization in osmolality and range of density of a continuous ficoll-sodium-diatrizoate gradient for isopycnic purification of isolated human islets.

  Eckhard M. et al, (2004), Transplant Proc, 36, 2849 - 2854

• Can the density of native pancreatic tissue slices predict human islet isolation and purification outcome?

  Eckhard M. et al, (2004), Transplant Proc, 36, 2845 - 2848

• The role of current product release criteria for identification of human islet preparations suitable for clinical transplantation.

  Eckhard M. et al, (2004), Transplant Proc, 36, 1528 - 1531

• Islet cell transplantation - Current state and perspectives

  Brendel MD. et al, (2003), Diabetes und Stoffwechsel, 12, 239 - 252

• Pretreatment of isolated islets with caspase-3 inhibitor DEVD increases graft survival after xenotransplantation.

  Brandhorst H. et al, (2003), Transplant Proc, 35

• Heat-exposed pig islets are protected from inflammatory mediators but express proapoptotic proteins.

  Brandhorst D. et al, (2003), Transplant Proc, 35

• Successful human islet isolation utilizing recombinant collagenase.

  Brandhorst H. et al, (2003), Diabetes, 52, 1143 - 1146

• Hyperthermic preconditioning protects pig islet grafts from early inflammation but enhances rejection in immunocompetent mice.

  Brandhorst D. et al, (2003), Cell Transplant, 12, 859 - 865

• Different role of saturated and unsaturated fatty acids in beta-cell apoptosis.

  Eitel K. et al, (2002), Biochem Biophys Res Commun, 299, 853 - 856

• High-dosed nicotinamide decreases early graft failure after pig to nude rat intraportal islet transplantation.

  Brandhorst D. et al, (2002), Transplantation, 73, 74 - 79

• Expression of complement regulatory proteins on islets of Langerhans: a comparison between human islets and islets isolated from normal and hDAF transgenic pigs.

  Bennet W. et al, (2001), Transplantation, 72, 312 - 319

• Experience with human islet transplantation in Sweden.

  Tibell A. et al, (2001), Transplant Proc, 33, 2535 - 2536

• Prevention of intraportal islet failure by a highly selective iNOS inhibitor in the pig-to-nude rat model.

  Brandhorst D. et al, (2001), Transplant Proc, 33

• Metabolic effects of restoring partial beta-cell function after islet allotransplantation in type 1 diabetic patients.

  Luzi L. et al, (2001), Diabetes, 50, 277 - 282

• Prevention of early islet graft failure by selective inducible nitric oxide synthase inhibitors after pig to nude rat intraportal islet transplantation.

  Brandhorst D. et al, (2001), Transplantation, 71, 179 - 184

• Islet transplantation: present clinical situation and future aspects.

  Bretzel RG. et al, (2001), Exp Clin Endocrinol Diabetes, 109 Suppl 2, S384 - S399

• Pretransplant induction of HSP-70 in isolated adult pig islets decreases early islet xenograft survival.

  Brandhorst D. et al, (2000), Cell Transplant, 9, 423 - 430

• Isolation of adult porcine islets of Langerhans.

  Toso C. et al, (2000), Cell Transplant, 9, 297 - 305

Key Publications

• Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.

  Brandhorst H. et al, (2013), Cell Transplant, 22, 1477 - 1483

• Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs.

  Asif S. et al, (2012), J Surg Res, 178, 959 - 967

• A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.

  Brandhorst H. et al, (2010), Transplantation, 89, 155 - 160

• Perfluorohexyloctane improves long-term storage of rat pancreata for subsequent islet isolation.

  Brandhorst H. et al, (2009), Transpl Int, 22, 1017 - 1022

• Pig pancreas oxygenation at 20 degrees C increases islet ATP generation but deteriorates islet function.

  Iken M. et al, (2009), Cell Transplant, 18, 745 - 751

Recent Publications

• Anti-caspase-3 preconditioning increases proinsulin secretion and deteriorates posttransplant function of isolated human islets.

  Brandhorst D. et al, (2013), Apoptosis, 18, 681 - 688

• Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.

  Brandhorst H. et al, (2013), Cell Transplant, 22, 1477 - 1483

• Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs.

  Asif S. et al, (2012), J Surg Res, 178, 959 - 967

• Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs

  Asif S. et al, (2012), Journal of Surgical Research, 178, 959 - 967

• Using HTK for prolonged pancreas preservation prior to human islet isolation.

  Caballero-Corbalán J. et al, (2012), J Surg Res, 175, 163 - 168