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- Oxford Academic Paediatric Surgery Unit (APSU) Research Group
Senior Postdoctoral Scientist
The success of human islet transplantation strongly depends on the outcome of the enzymatic islet isolation process. My research activity aims at optimisation and standarisation of protease blends to maximise islet release from within the acinar tissue of the human pancreas and to prevent enzymatic destruction of these fragile micro-organs.
As an initial step toward enzyme standardisation we previously provided the prove of principal that successful human islet isolation is possible by utilisation of a recombinant collagenase blend.
Further optimsation was obtained by identifying the ideal ratio between collagenase class I and class II which was an important step to reduce islet-toxic proteolytic activities.
Currently we are working to identify alternative enzymes that can replace neutral protease activities which are characterised by islet-toxicity.
Anti-caspase-3 preconditioning increases proinsulin secretion and deteriorates posttransplant function of isolated human islets.
Brandhorst D. et al, (2013), Apoptosis, 18, 681 - 688
Quality of isolated pig islets is improved using perfluorohexyloctane for pancreas storage in a split lobe model.
Brandhorst H. et al, (2013), Cell Transplant, 22, 1477 - 1483
Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs.
Asif S. et al, (2012), J Surg Res, 178, 959 - 967
Oxygen-charged HTK-F6H8 emulsion reduces ischemia-reperfusion injury in kidneys from brain-dead pigs
Asif S. et al, (2012), Journal of Surgical Research, 178, 959 - 967
Using HTK for prolonged pancreas preservation prior to human islet isolation.
Caballero-Corbalán J. et al, (2012), J Surg Res, 175, 163 - 168