Senior Postdoctoral Scientist
The success of human islet transplantation strongly depends on the outcome of the enzymatic islet isolation process. My research activity aims at optimisation and standarisation of protease blends to maximise islet release from within the acinar tissue of the human pancreas and to prevent enzymatic destruction of these fragile micro-organs.
As an initial step toward enzyme standardisation we previously provided the prove of principal that successful human islet isolation is possible by utilisation of a recombinant collagenase blend.
Further optimsation was obtained by identifying the ideal ratio between collagenase class I and class II which was an important step to reduce islet-toxic proteolytic activities.
Currently we are working to identify alternative enzymes that can replace neutral protease activities which are characterised by islet-toxicity.
High Concentrations of Etanercept Reduce Human Islet Function and Integrity
Brandhorst D. et al, (2021), Journal of Inflammation Research, Volume 14, 599 - 610
Proteomic Profiling Reveals the Ambivalent Character of the Mesenchymal Stem Cell Secretome: Assessing the Effect of Preconditioned Media on Isolated Human Islets.
Brandhorst H. et al, (2020), Cell Transplant, 29
Strategies to improve the oxygen supply to microencapsulated islets.
Brandhorst D. et al, (2019), Transplantation
Comparison of Clostripain and Neutral Protease as Supplementary Enzymes for Human Islet Isolation.
Brandhorst H. et al, (2018), Cell Transplant
Does Islet Size Really Influence Graft Function After Clinical Islet Transplantation?
Hughes SJ. et al, (2018), Transplantation, 102, 1857 - 1863