Senior Postdoctoral Scientist
The success of human islet transplantation strongly depends on the outcome of the enzymatic islet isolation process. My research activity aims at optimisation and standarisation of protease blends to maximise islet release from within the acinar tissue of the human pancreas and to prevent enzymatic destruction of these fragile micro-organs.
As an initial step toward enzyme standardisation we previously provided the prove of principal that successful human islet isolation is possible by utilisation of a recombinant collagenase blend.
Further optimsation was obtained by identifying the ideal ratio between collagenase class I and class II which was an important step to reduce islet-toxic proteolytic activities.
Currently we are working to identify alternative enzymes that can replace neutral protease activities which are characterised by islet-toxicity.
Does islet size really influence graft function following clinical islet transplantation?
Hughes SJ. et al, (2018), Transplantation
Hypoxia-Induced Damage in Human Islets Is Reduced With the Use of Mesenchymal Stem Cell-Preconditioned Medium.
Brandhorst D. et al, (2017), Transplant Proc, 49, 2330 - 2332
Effect of Etanercept Concentration on Human Islet Integrity.
Acreman S. et al, (2017), Transplant Proc, 49, 2327 - 2329
Quantifying the Effects of Different Neutral Proteases on Human Islet Integrity.
Brandhorst H. et al, (2017), Cell Transplant, 26, 1733 - 1741
Enzyme development for human islet isolation: Five decades of progress or stagnation?
Brandhorst D. et al, (2017), Review of Diabetic Studies, 14, 22 - 38