Oxford Centre for Diabetes, Endocrinology and Metabolism (OCDEM), Churchill Hospital, Oxford OX3 7LE
Senior Postdoctoral Scientist
The success of human islet transplantation strongly depends on the outcome of the enzymatic islet isolation process. My research activity aims at optimisation and standarisation of protease blends to maximise islet release from within the acinar tissue of the human pancreas and to prevent enzymatic destruction of these fragile micro-organs.
As an initial step toward enzyme standardisation we previously provided the prove of principal that successful human islet isolation is possible by utilisation of a recombinant collagenase blend.
Further optimsation was obtained by identifying the ideal ratio between collagenase class I and class II which was an important step to reduce islet-toxic proteolytic activities.
Currently we are working to identify alternative enzymes that can replace neutral protease activities which are characterised by islet-toxicity.
The ischaemic preconditioning paradox and its implications for islet isolation from heart-beating and non heart-beating donors.
Brandhorst D. et al, (2022), Sci Rep, 12
A highly oxygenated hydrogel enhanced the survival of human islets encapsulated within macroencapsulation devices
Domingo-Lopez DA. et al, (2022), DIABETOLOGIA, 65, S92 - S92
Comparison of different hyaluronic acid-based matrices to maintain human islet survival in macroencapsulation devices
Brandhorst D. et al, (2022), DIABETOLOGIA, 65, S198 - S199
Basement membrane proteins improve human islet survival in hypoxia: Implications for islet inflammation.
Brandhorst D. et al, (2022), Acta Biomater, 137, 92 - 102
High Concentrations of Etanercept Reduce Human Islet Function and Integrity.
Brandhorst D. et al, (2021), J Inflamm Res, 14, 599 - 610