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We are currently undertaking research to determine how regulatory T cells function in vivo to control transplant rejection. It is established that regulatory T cell presence within the allograft is important for control of rejection.  However, our understanding where Treg are acting to exert this control is limited. We are developing strategies and techniques to use multi-photon microscopy to allow us to observe regulatory T cell interactions within the allograft and subsequently determine both how they control alloreactivity and the effects of the surrounding environment on their ability to achieve this.